Journal: Gels

Article Title: Tissue Regeneration with Gelatine/Polysaccharide Derived Hydrogel Scaffolds: From Formulation to In Vivo Efficacy

doi: 10.3390/gels9090744

Figure Lengend Snippet: Vascularisation analysis of wound tissues. ( A ). Immunofluorescence of CD31, and α-SMA. Scale bar: 20 μm. ( B ). Quantitative analysis of CD31-positive stained immature vessels. ( C ). Quantitative analysis of both CD31- and α-SMA-positive stained mature vessels. * p < 0.05, ** p < 0.01, NS, no significant difference.

Article Snippet: Then, the sections were blocked by serum and incubated with anti-CD31 primary antibody (Abbiotec, San Diego, CA, USA) and anti-α-SMA antibody (Abcam, Cambridge, UK).

Techniques: Immunofluorescence, Staining

Journal: Brain, behavior, and immunity

Article Title: Bone marrow mononuclear cells exert long-term neuroprotection in a rat model of ischemic stroke by promoting arteriogenesis and angiogenesis

doi: 10.1016/j.bbi.2013.07.010

Figure Lengend Snippet: Transplanted BMMNCs incorporate into brain vessels and promote angiogenesis. Representative images of leptomeningeal anastomoses on days 14, 28 and 42 (A–I) and intracranial parenchymal microvessels on day 42 (J–L) stained with anti-BrdU antibody (green) and anti-CD31 antibody (red) (scale bar = 50 μm). (M–N) Quantification showed an increase in the number of BrdU-positive endothelial cells (ECs, M) and smooth muscle cells (SMCs, N). *p<0.05 vs. the number of BrdU-positive ECs or SMCs on day 14 or 42; n=6/time point.

Article Snippet: Mouse anti-BrdU monoclonal antibody (BBI, Shanghai, China), rabbit anti-rat polyclonal antibody against smooth muscle actin (α-SMA; BBI), and rabbit anti-rat polyclonal antibody against CD31 (BBI) were used as primary antibodies at a dilution of 1:250.

Techniques: Staining